Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/67854
標題: PORTABLE MICROSATELLITE PRIMERS FOR FICUS (MORACEAE)
作者: Garcia, M.
Bain, A.
Tzeng, H.Y.
Peng, Y.Q.
Chou, L.S.
Kjellberg, F.
關鍵字: coevolution
Ficus
Moraceae
mutualism
figs
期刊/報告no:: American Journal of Botany, Volume 99, Issue 5, Page(s) E187-E192.
摘要: Premise of the study: Highly portable microsatellite primers were developed for Ficus to facilitate investigation of genetic structure of complete regional floras using a single set of markers. Methods and Results: Pyrosequencing of five species of Ficus produced a library of 5723 potential primers. Potential primers found in at least two species and presenting identical annealing temperatures were tested on a set of five additional Ficus species. A set of 20 primer pairs producing well-defined and easily readable peaks was retained and tests showed their potential utility for analyzing population genetic structure of 24 Ficus species from Taiwan. Numbers of alleles per locus ranged from one to six in the least variable species and from one to 17 in the most variable species. Conclusions: The results indicate that our set of primers can be used to analyze polymorphism and compare levels of polymorphism among Ficus species.
URI: http://hdl.handle.net/11455/67854
ISSN: 0002-9122
文章連結: http://dx.doi.org/10.3732/ajb.1100485
Appears in Collections:期刊論文

文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.