Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/67926
標題: Characterization of AFLP sequences from regions of maize B chromosome defined by 12 B-10L translocations
作者: Peng, S.F.
Lin, Y.P.
Lin, B.Y.
關鍵字: molecular analysis
markers
origin
nondisjunction
locus
rflp
期刊/報告no:: Genetics, Volume 169, Issue 1, Page(s) 375-388.
摘要: Maize B chromosome sequences have been previously cloned by microdissection, and all are proven to be highly repetitive, to be homologous to the normal complement, and to show no similarity to any published gene other than mobile elements. In this study, we isolated sequences from defined B regions. The strategy involved identification and then mapping of AFLP-derived B fragments before cloning. Of 14 B AFLPs, 13 were mapped by 12 B-10L translocations: 3 around the centromeric knob region, 3 in the proximal euchromatic, I around the border of proximal euchromatic and distal heterochromatic, and 6 in the distal heterochromatic region of the B long arm. The AFLP fragments were cloned and sequenced. Analogous to the microdissected sequences, all sequences were repetitive, and all but two were highly homologous to the A chromosomes. FISH signals of all but three clones appeared in pachytene B as well as in somatic A and B chromosomes. None of these clones exhibits identity to any published gene. Six clones displayed homology to two centromeric BACs, four to sequences of chromosomes 3, 4, 7, and 10, four to retrotransposons, and three to no sequence deposited in GenBank. Furthermore, flanking regions of two highly B-specific clones were characterized, showing extension of a B-exclusive nature. The possibility of the presence of novel B repeat(s) is discussed.
URI: http://hdl.handle.net/11455/67926
ISSN: 0016-6731
文章連結: http://dx.doi.org/10.1534/genetics.104.032417
Appears in Collections:期刊論文

文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.