請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/67953
標題: Curcumin Induces Apoptosis in Human Non-small Cell Lung Cancer NCI-H460 Cells through ER Stress and Caspase Cascade- and Mitochondria-dependent Pathways
作者: Wu, S.H.
Hang, L.W.
Yang, J.S.
Chen, H.Y.
Lin, H.Y.
Chiang, J.H.
Lu, C.C.
Yang, J.L.
Lai, T.Y.
Ko, Y.C.
Chung, J.G.
關鍵字: Curcumin
apoptosis
G(2)/M arrest
caspase cascade
endoplasmic
reticulum stress
lung neoplasms
herbal medicine
endoplasmic-reticulum stress
cytochrome-c
a-549 cells
fas ligand
in-vivo
activation
death
arrest
phase
dna
期刊/報告no:: Anticancer Research, Volume 30, Issue 6, Page(s) 2125-2133.
摘要: It has been reported that curcumin inhibited various types of cancer cells in vitro and in vivo. However, mechanisms of curcumin-inhibited cell growth and -induced apoptosis in human non-small cell lung cancer cells (NCI-H460) still remain unclear. In this study, NCI-H460 cells were treated with curcumin to determine its anticancer activity. Different concentrations of curcumin were used for different durations in NCI-H460 cells and the subsequent changes in the cell morphology, viability, cell cycle, mRNA and protein expressions were determined. Curcumin induced apoptotic morphologic changes in NCI-H460 cells in a dose-dependent manner. After curcumin treatment, BAX and BAD were up-regulated, BCL-2, BCL-X(L) and XIAP were down-regulated. In addition, reactive oxygen species (ROS), intracellular Ca(2+) and endoplasmic reticulum (ER) stress were increased in NCI-H460 cells after exposure to curcumin. These signals led to a loss of mitochondrial membrane potential (Delta Psi(m)) and culminated in caspase-3 activation. Curcumin-induced apoptosis was also stimulated through the FAS/caspase-8 (extrinsic) pathway and ER stress proteins, growth arrest- and DNA damage-inducible gene 153 (GADD153) and glucose-regulated protein 78 (GRP78) were activated in the NCI-H460 cells. Apoptotic cell death induced by curcumin was significantly reversed by pretreatment with ROS scavenger or caspase-8 inhibitor. Furthermore, the NCI-H460 cells tended to be arrested at the G(2)/M cell cycle stage after curcumin treatment and down-regulation of cyclin-dependent kinase 1 (CDK1) may be involved. In summary, curcumin exerts its anticancer effects on lung cancer NCI-H460 cells through apoptosis or cell cycle arrest.
URI: http://hdl.handle.net/11455/67953
ISSN: 0250-7005
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