Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/67956
標題: Houttuynia cordata Thunb Extract Inhibits Cell Growth and Induces Apoptosis in Human Primary Colorectal Cancer Cells
作者: Lai, K.C.
Chiu, Y.J.
Tang, Y.J.
Lin, K.L.
Chiang, J.H.
Jiang, Y.L.
Jen, H.F.
Kuo, Y.H.
Agamaya, S.
Chung, J.G.
Yang, J.S.
關鍵字: Houttuynia cordata Thunb (HCT)
chinese herb
apoptosis
human primary
colorectal cancer cells
mitochondrial-dependent pathway
death pathway
model
expression
therapy
hl-60
期刊/報告no:: Anticancer Research, Volume 30, Issue 9, Page(s) 3549-3556.
摘要: It is reported that Houttuynia cordata Thunb. (HCT), a traditional Chinese herbal medicine, has many biological properties such as antiviral, antibacterial and antileukemic activities. However, the molecular mechanisms of cytotoxicity and apoptosis in human primary colorectal cancer cells are not clear. In this study, whether HCT induced cytotoxicity in primary colorectal cancer cells obtained from three patients was investigated. The results indicated that HCT inhibited growth of cancer cells in a dose-dependent manner. After treatment with HCT (250 mu g/ml) for 24 h, cells exhibited chromatin condensation (an apoptotic characteristic). HCT increased reactive oxygen species (ROS) production and decreased the mitochondrial membrane potential (Delta Psi(m)) in examined cells. Mitochondria-dependent apoptotic signaling pathway was shown to be involved as determined by increase in the levels of cytochrome c, Apaf-1, and caspase-3 and -9. The decrease in the level of Delta Psi(m) was associated with an increase in the BAX/BCL-2 ratio which led to activation of caspase-9 and -3. Based on our results, HCT induced apoptotic cell death in human primary colorectal cancer cells through a mitochondria-dependent signaling pathway.
URI: http://hdl.handle.net/11455/67956
ISSN: 0250-7005
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