Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/68099
標題: Protective effects of magnolol against oxidized LDL-induced apoptosis in endothelial cells
作者: Ou, H.C.
Chou, F.P.
Sheu, W.H.H.
Hsu, S.L.
Lee, W.J.
關鍵字: endothelium
OxLDL
magnolol
apoptosis
mitochondrial membrane
potential
calcium homeostasis
low-density-lipoprotein
nf-kappa-b
oxygen species production
laser-scanning cytometry
smooth-muscle cells
transcription factor
in-vitro
ca2+ mobilization
honokiol
activation
期刊/報告no:: Archives of Toxicology, Volume 81, Issue 6, Page(s) 421-432.
摘要: Magnolol, a compound extracted from the Chinese medicinal herb Magnolia officinalis, has several biological effects. However, its protective effects against endothelial injury remain unclear. In this study, we examined whether magnolol prevents oxidized low density lipoprotein (oxLDL)-induced vascular endothelial apoptosis. Incubation of oxLDL with magnolol (2.5-20 mu M) inhibited copper-induced oxidative modification via diene formation, thiobarbituric acid reactive substances (TBARS) assay and electrophoretic mobility assay. Apoptotic cell death as characterized by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) stain. We measured the production of reactive oxygen species (ROS) by using the fluorescent probe 2 ',7 '-dichlorofluorescein acetoxymethyl ester (DCF-AM), and observed the activity of antioxidant enzymes. Furthermore, several apoptotic signaling pathways which showed NF-kappa B activation, increased cytosolic calcium, alteration of mitochondrial membrane potential, cytochrome c release and activation of caspase 3 were also investigated. We demonstrated that magnolol prevented the copper-induced oxidative modification of LDL. Magnolol attenuated the oxLDL-induced ROS generation and subsequent NF-kappa B activation. Furthermore, intracellular calcium accumulation and subsequent mitochondrial membrane potential collapse, cytochome c release and activation of caspase 3 caused by oxLDL were also inhibited by magnolol. Our results suggest that magnolol may have clinical implications in the prevention of atherosclerotic vascular disease through decreasing the oxLDL-induced ROS production.
URI: http://hdl.handle.net/11455/68099
ISSN: 0340-5761
文章連結: http://dx.doi.org/10.1007/s00204-006-0172-3
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