Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/68103
標題: Genetic analysis of fish iridoviruses isolated in Taiwan during 2001-2009
作者: Huang, S.M.
Tu, C.
Tseng, C.H.
Huang, C.C.
Chou, C.C.
Kuo, H.C.
Chang, S.K.
關鍵字: major capsid protein
red-sea bream
ranavirus family iridoviridae
lymphocystis disease virus
complete genome sequence
grouper
iridovirus
phylogenetic analysis
ornamental fish
molecular
epidemiology
pcr amplification
期刊/報告no:: Archives of Virology, Volume 156, Issue 9, Page(s) 1505-1515.
摘要: To investigate the genetic relationships between field strains of iridoviruses gathered from various fish species in Taiwan, viruses that were collected from 2001 to 2009 were analyzed. Open reading frames encoding the viral major capsid protein (MCP) and adenosine triphosphatase (ATPase) were sequenced for phylogenetic analysis. Our results indicated that iridoviruses from Taiwan aquaculture fishes could be classified into two groups: prior to 2005, the viruses were closely related to members of the genus Ranavirus; and after 2005, they were similar to members of the genus Megalocytivirus. Based on the analysis of MCP amino acid sequences, virus isolates were divided into 4 major genotypes that were related to ISKNV, RSIV, FLIV, and GIV, respectively. Pairwise comparisons of MCP genes showed that the ranavirus was an epidemic pathogen for economically important species in the major production regions and cultured marine fish, while the megalocytivirus isolates were sensitive to host range. In addition, the distribution of synonymous and non-synonymous changes in the MCP gene revealed that the iridoviruses were evolving slowly, and most of the variations were synonymous mutations. The Ka/Ks values were lower than one, and hence, the viruses were under negative selection.
URI: http://hdl.handle.net/11455/68103
ISSN: 0304-8608
文章連結: http://dx.doi.org/10.1007/s00705-011-1017-9
Appears in Collections:期刊論文

文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.