Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/68151
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dc.contributor.authorChang, C.C.en_US
dc.contributor.authorChuang, Y.C.en_US
dc.contributor.authorChen, Y.C.en_US
dc.contributor.authorChang, M.C.en_US
dc.date2004zh_TW
dc.date.accessioned2014-06-11T05:56:19Z-
dc.date.available2014-06-11T05:56:19Z-
dc.identifier.issn0006-291Xzh_TW
dc.identifier.urihttp://hdl.handle.net/11455/68151-
dc.description.abstractA blue fluorescent protein, BfgV, belonging to the short-chain dehydrogenase/reductase superfamily, was found in a non-bioluminescent pathogen Vibrio vulnificus CKM-1. This protein has fluorescence spectra with two excitation peaks at 283 and 352 nm, and one emission peak at 456 nm. BfgV fluoresces through effectively augmenting the intrinsic fluorescence of NADPH bound to it. Escherichia coli transformants expressing this protein can emit eye-detectable fluorescence. A LysR-type transcriptional regulator gene bfgR was found at the vicinal upstream region of bfgV in CKM-1 genome. The clues that products of bfgR can specifically bind to bfgR-bfgV intergenic promoter region and the deletion of bfgR significantly decreases the expression of bfgV reveal bfgR is a repressor gene of bfgV in V. vulnificus CKM-1. (C) 2004 Elsevier Inc. All rights reserved.en_US
dc.language.isoen_USzh_TW
dc.relationBiochemical and Biophysical Research Communicationsen_US
dc.relation.ispartofseriesBiochemical and Biophysical Research Communications, Volume 319, Issue 1, Page(s) 207-213.en_US
dc.relation.urihttp://dx.doi.org/10.1016/j.bbrc.2004.04.168en_US
dc.subjectfluorescenceen_US
dc.subjectNADPHen_US
dc.subjectshort-chain dehydrogenase/reductaseen_US
dc.subjectVibrioen_US
dc.subjectvulnificusen_US
dc.subjectshort-chain dehydrogenasesen_US
dc.subjectbacterial bioluminescenceen_US
dc.subjectmolecular-biologyen_US
dc.subjectcoenzyme bindingen_US
dc.subjectconstructionen_US
dc.subjectluminescenceen_US
dc.subjectpolymeraseen_US
dc.subjectpromotersen_US
dc.subjectmechanismen_US
dc.subjectsystemen_US
dc.titleBright fluorescence of a novel protein from Vibrio vulnificus depends on NADPH and the expression of this protein is regulated by a LysR-type regulatory geneen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1016/j.bbrc.2004.04.168zh_TW
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