請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/68162
標題: Medium optimization and production of secreted Renilla luciferase in Bacillus subtilis by fed-batch fermentation
作者: Chen, P.T.
Chiang, C.J.
Chao, Y.P.
關鍵字: Renilla luciferase
Experimental design
Fed-batch fermentation
Bacillus subtilis
response-surface methodology
recombinant nattokinase
molecular-cloning
expression
vector
instability
stability
plasmids
proteins
pub110
期刊/報告no:: Biochemical Engineering Journal, Volume 49, Issue 3, Page(s) 395-400.
摘要: Luciferase of the soft coral Renilla reniformis (Rluc) has found a multitude of potential applications. However, its production in a secreted form has been solely performed in mammalian cells. In this work, a high production of secreted Rluc was approached in Bacillus subtilis by implementing the fermentation strategy. First, three critical medium components among others were selected by the Plackett-Burman design. Followed by the Box-Behnken design, the medium formulation was optimized and composed of 1.17% glucose, 2.27% yeast extract, and 0.55% glutamate. With the optimum medium, the shake-flask culture of the producer strain was carried out to secret Rluc with the yield of 55 mg/L. Finally, the production scheme was scaled up using a 5-L jar fermenter operated in a fed-batch mode. By controlling the operational condition, the feeding solution was pumped in an impulse mode to the culture. As a consequence, the production of Rluc reaching 500 mg/L was obtained after fermentation for 27 h. (C) 2010 Elsevier B.V. All rights reserved.
URI: http://hdl.handle.net/11455/68162
ISSN: 1369-703X
文章連結: http://dx.doi.org/10.1016/j.bej.2010.02.001
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