Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/68188
標題: Effects of ketoconazole on progesterone and cAMP production in MA-10 mouse Leydig tumor cells
作者: Chang, C.L.T.
Fung, H.P.
關鍵字: ketoconazole
progesterone
cAMP
Leydig cell
high-dose ketoconazole
adrenal steroidogenesis
testicular function
inhibition
mechanism
biosynthesis
enzymes
invitro
invivo
dogs
期刊/報告no:: Biological & Pharmaceutical Bulletin, Volume 25, Issue 6, Page(s) 794-797.
摘要: The effects of ketoconazole (KCZ) on secretion of progesterone and cAMP in Leydig cells were investigated in vitra. MA-10 mouse Leydig tumor cells were used to conduct the experiments. KCZ significantly inhibited the progesterone production from MA-10 cells in a dose dependent fashion between 2 and 20 mum among 1, 2 and 3 h of incubation. There was a statistically significant difference in luteinizing hormone (LH) stimulated progesterone production inhibited by 2 and 20 mum KCZ treatment compared to the control. The effect of KCZ on progesterone biosynthesis in MA-10 cells was mediated by cAMP. since KCZ suppressed basal and LH stimulated cAMP production and content within the same dose range. The stimulatory effects of forskolin and sodium fluoride on the aden late cyclase system were also inhibited by KCZ. Moreover. dibutyryl cAMP blocked the inhibitory effect on steroidogenesis of KCZ in MA-10 cells. These data indicated that KCZ induced the inhibition of a catalytic component of adenylate cyclase holoenzyme in MA-10 mouse Leydig tumor cells.
URI: http://hdl.handle.net/11455/68188
ISSN: 0918-6158
文章連結: http://dx.doi.org/10.1248/bpb.25.794
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