Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/68220
標題: LOW-INTENSITY ULTRASOUND INCREASES THE ENDOTHELIAL NITRIC OXIDE SYNTHASE (eNOS) EXPRESSION OF ENDOTHELIAL CELLS POSSIBLY VIA THE PHOSPHATIDYLINOSITOL 3-KINASE/AKT/PROTEIN KINASE A SIGNALING PATHWAY
作者: Hsu, S.H.
Huang, T.B.
Wang, K.P.
關鍵字: Ultrasound
Endothelial cells
Endothelial nitric oxide synthase
in-vitro
therapeutic ultrasound
human fibroblasts
rat chondrocytes
bone-formation
osteoblasts
phosphorylation
activation
3-kinase
calcium
期刊/報告no:: Biomedical Engineering-Applications Basis Communications, Volume 22, Issue 5, Page(s) 367-376.
摘要: The stimulative effects and the associated mechanism of low-intensity ultrasound on endothelial cells were investigated in this study. Human umbilical cord endothelial cells (HUVECs) were exposed to 1 MHz pulsed ultrasound at 34 mW/cm(2) for 10 min daily. The cell proliferation, nitric oxide release, intracellular calcium concentration, and endothelial nitric oxide synthase (eNOS) gene and protein expressions after ultrasound exposure were analyzed. It was found that ultrasound exposure did not change the cell proliferation, but it enhanced the release of nitric oxide and Ca(2+) ions, and increased the eNOS activity. The induced phosphorylation of eNOS (Ser(1177)) by the ultrasound exposure was inhibited by the addition of N-[ 2-(p-bromocinnamylamino) ethyl]-5-isoquinoline sulfonamide (H89, a protein kinase A inhibitor), but not 2-(4-morpholino)-8-phenyl-4H-1-benzopyran-4-one hydrochloride (LY294002, a phosphoatidylinositol 3-kinase inhibitor). The induced phosphorylation of Akt (protein kinase B, Ser(473)) by the ultrasound exposure was inhibited by the addition of LY294002, but not by the presence of H89. The results also applied to bovine carotid artery endothelial cells (BAECs) exposed to ultrasound at a larger intensity (68 mW/cm(2)). The above experiments suggested that low-intensity ultrasound may promote eNOS activity of endothelial cells via the phosphatidylinositol 3-kinase/Akt/protein kinase A signaling pathway. This may have implications on the therapeutic effect of ultrasound in tissue repair.
URI: http://hdl.handle.net/11455/68220
ISSN: 1016-2372
文章連結: http://dx.doi.org/10.4015/s1016237210002122
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