Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/68256
DC FieldValueLanguage
dc.contributor.authorChen, C.Y.en_US
dc.contributor.authorHsieh, J.L.en_US
dc.contributor.authorSilver, S.en_US
dc.contributor.authorEndo, G.en_US
dc.contributor.authorHuang, C.C.en_US
dc.date2008zh_TW
dc.date.accessioned2014-06-11T05:56:31Z-
dc.date.available2014-06-11T05:56:31Z-
dc.identifier.issn0916-8451zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/68256-
dc.description.abstractThe mercury resistance module of Bacillus transposon TnMERI1 is regulated by three operator/promoter regions (O/P(merB3), O/P(merR1), and O/P(merR2)) and two regulatory proteins (MerR1 and MerR2) encoded by the module itself. To clarify the roles of MerR1 and MerR2 in the regulatory mechanism, both proteins were overexpressed and purified. MerR1 bound the regulatory regions O/P(merB3) and O/P(merR1), with a preference for O/P(merB3) as measured on in vitro gel shift assays. However, TN4erR2 bound OlPmerR29 as revealed by gel shift and restriction endonuclease protection assays. The transcriptional start sites of O/P(merB3) and O/P(merR2) were determined by rapid amplification of 5'-cDNA ends (5'-RACE) in the TnMERI1 original host, Bacillus megaterium strain MB1. Real-time reverse transcription polymerase chain reaction (RT-PCR) assays showed that O/P(merB3) and O/P(merR1) were induced in the presence of Hg(2+) but not O/P(merR2). It was concluded that MerR1 regulates O/P(merB3) and O/P(merR1), while MerR2 regulates O/P(merR2).en_US
dc.language.isoen_USzh_TW
dc.relationBioscience Biotechnology and Biochemistryen_US
dc.relation.ispartofseriesBioscience Biotechnology and Biochemistry, Volume 72, Issue 9, Page(s) 2403-2410.en_US
dc.relation.urihttp://dx.doi.org/10.1271/bbb.80294en_US
dc.subjectBacillus megateriumen_US
dc.subjectmer operonen_US
dc.subjectMerRen_US
dc.subjectgram-positive bacteriaen_US
dc.subjectclass-ii transposonen_US
dc.subjectnatural environmentsen_US
dc.subjectminamata bayen_US
dc.subjectmetalen_US
dc.subjectdnaen_US
dc.subjecttranscriptionen_US
dc.subjectoperonsen_US
dc.subjectgenesen_US
dc.subjectjapanen_US
dc.titleInteractions between two MerR regulators and three operator/promoter regions in the mercury resistance module of Bacillus megateriumen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1271/bbb.80294zh_TW
Appears in Collections:期刊論文
文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.