請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/68258
標題: Overexpression of a single membrane component from the Bacillus mer operon enhanced mercury resistance in an Escherichia coli host
作者: Hsieh, J.L.
Chen, C.Y.
Chang, J.S.
Endo, G.
Huang, C.C.
關鍵字: MerP
mercury
bioremediation
ion-binding protein
class-ii transposon
p-type atpase
metal-binding
bacterial resistances
cysteine residues
heavy-metals
expression
peptides
cells
期刊/報告no:: Bioscience Biotechnology and Biochemistry, Volume 71, Issue 6, Page(s) 1494-1499.
摘要: Overexpression of a mercuric ion binding protein, MerP, from the mercury resistance operon genes of Gram-positive bacterial strain Bacillus megaterium MB1 and from Gram-negative bacterial strain Pseudomonas aeruginosa K-62 was found to enhance the mercury resistance level of Escherichia coli host cells, even though they share only 27.3% identity. Immunoblot analysis showed that MerP (BMerP) from Bacillus could be expressed on the membrane fraction of E. coli cells. Treated with 10 mu M Hg2+, a recombinant strain harboring the BMerP gene significantly improved, showing a 27% increase in mercuric ion adsorption capacity, 16% better than that of a Pseudomonas merP gene (PMerP)-harboring strain. While multiple heavy metals co-existed, the mercuric ion adsorption capacity of the BMerP-harboring E. coli was not affected while that of the PMerP-harboring strain decreased. These results suggest that BMerP can act as a bio-adsorbent compartmentalizing the toxic mercuric ion on the cell membrane and enhancing resistance.
URI: http://hdl.handle.net/11455/68258
ISSN: 0916-8451
文章連結: http://dx.doi.org/10.1271/bbb.70003
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