Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/68259
標題: Simultaneous detection of NADH and H2O2 using flow injection analysis based on a bifunctional poly(thionine)-modified electrode
作者: Baskar, S.
Chang, J.L.
Zen, J.M.
關鍵字: Poly(thionine)
Preanodized screen printed carbon electrode
NADH
Hydrogen peroxide
screen-printed electrodes
hydrogen-peroxide
carbon electrodes
oxidation
dehydrogenase
biosensor
oxidase
thionine
system
sites
期刊/報告no:: Biosensors & Bioelectronics, Volume 33, Issue 1, Page(s) 95-99.
摘要: We report here a novel detection scheme for simultaneous detection of NADH and H2O2 based on a bifunctional poly(thionine)-modified electrode. Electropolymerization of thionine on a "preanodized" screen-printed carbon electrode effectively lowers the oxidation potential of NADH to 0.15 V (vs. Ag/AgCl). Since poly(thionine) is also a well known electrochemical mediator for H2O2 reduction, we further developed a poly(thionine)-modified ring disk electrode for simultaneous measurement of nicotinamide adenine dinucleotide (NADH) and hydrogen peroxide (H2O2) by flow injection analysis. By applying the optimized detection potentials of 0.2 V and -0.2 V at disk and ring electrodes, respectively, this system allows the simultaneous measurement of both analytes with good sensitivity (0.13 mu A/mM for H2O2 and 0.34 mu A/mM for NADH) and limit of detection (1.74 mu M and 26.0 mu M for NADH and H2O2). This opens the possibility of a whole series of biosensor applications. (C) 2011 Elsevier B.V. All rights reserved.
URI: http://hdl.handle.net/11455/68259
ISSN: 0956-5663
文章連結: http://dx.doi.org/10.1016/j.bios.2011.12.025
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