請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/68300
標題: An assessment of genetic relationships in cultivated tea clones and native wild tea in Taiwan using RAPD and ISSR maskers
作者: Lai, J.A.
Yang, W.C.
Hsiao, J.Y.
關鍵字: AMOVA
Camellia sinensis
genetic variation
ISSR
RAPD
tea
sequence repeat polymorphisms
dna polymorphisms
diversity
markers
identification
poaceae
bambusoideae
populations
l.
期刊/報告no:: Botanical Bulletin of Academia Sinica, Volume 42, Issue 2, Page(s) 93-100.
摘要: Tea (Camellia sinensis [L.] O. Kuntze) is an important beverage crop in Taiwan. Most of the cultivated clones were introduced from China and India though some native wild teas are distributed in the mountains of central and southern Taiwan. in this study, 37 tea samples were evaluated using RAPD and ISSR markers. The samples comprised 21 clones of China, 3 clones of Assam, 7 hybrid clones between China and Assam tea? and 6 individual samples of native Taiwanese wild tea. A total of 53 and 56 polymorphic RAPD and ISSR markers respectively, were scored. The results of cluster analysis based on RAPDs revealed that three major groups could he recognized, i.e,, cultivars of China tea and the cultivars developed in Taiwan from hybridization and selection; Assam tea; native Taiwanese wild tea. The native Taiwanese wild teas were, however, most distant in the clustering tree, In the ISSR dendrogram. Taiwanese native wild teas clustered closely with Assam tea then with China tea and the Taiwanese hybrid cultivars. The population gene diversity of the native wild tea was found to be the highest among the three populations studied. Analysis of molecular variance (AMOVA) revealed that the variance component within groups was larger than that among groups. The correlation coefficient between similarity matrices based on RAPD and ISSR was 0.811. A Mantel test revealed that the correlation was highly significant (p <0.001), indicating good congruence between the results of these two molecular markers.
URI: http://hdl.handle.net/11455/68300
ISSN: 0006-8063
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