Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/68367
標題: Cigarette smoking induces overexpression of hepatocyte growth factor in type II pneumocytes and lung cancer cells
作者: Chen, J.T.
陳鴻震
Lin, T.S.
Chow, K.C.
Huang, H.H.
Chiou, S.H.
Chiang, S.F.
Chen, H.C.
Chuang, T.L.
Lin, T.Y.
Chen, C.Y.
邱繡河
周寬基
關鍵字: immunohistochemistry
in situ hybridization
microarray
suppression
subtractive hybridization
type II pneumocyte
epithelial-cells
met protooncogene
primary culture
in-vivo
expression
gene
receptors
identification
pathway
injury
期刊/報告no:: American Journal of Respiratory Cell and Molecular Biology, Volume 34, Issue 3, Page(s) 264-273.
摘要: We examined gene expression of hepatocyte growth factor (HGF) and HGF receptor (HGFR), or product of proto-oncogene c-met (c-met), in smokers and nonsmokers with adenocarcinoma (ADC) by suppression subtractive hybridization and microarray techniques. Expression of HGF and c-met was confirmed by RT-PCR. HGF content in the respective tumor mass and nontumor lung tissue was measured by ELISA. HGF in pathologic samples was localized by immunohistochemistry and in situ hybridization. Our results indicate that overexpression of HGFR was frequently detected in ADC cells, whereas overexpression of HGF was detected in alveolar type II (ATII) cells. Overexpression of HGF was correlated with cigarette smoking and tumor stages. In vitro, HGF expression was evaluated in isolated murine ATII cells and in 12 ADC cell lines, and we found that nicotine activated HGF expression in ATII cells and lung cancer cells.
URI: http://hdl.handle.net/11455/68367
ISSN: 1044-1549
文章連結: http://dx.doi.org/10.1165/rcmb.2005-0117OC
Appears in Collections:生命科學院

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