Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/68376
標題: The anti-metastatic efficacy of beta-ionone and the possible mechanisms of action in human hepatocarcinoma SK-Hep-1 cells
作者: Huang, C.S.
Lyu, S.C.
Chen, J.Y.
Hu, M.L.
關鍵字: beta-Ionone
Metastasis
Nm23-H1
Focal adhesion kinase
Rho GTPase
focal adhesion kinase
hepatocellular-carcinoma cells
cancer-cells
signal-transduction
rho-gtpases
in-vitro
metastasis
migration
invasion
suppressor
期刊/報告no:: British Journal of Nutrition, Volume 107, Issue 5, Page(s) 631-638.
摘要: beta-Ionone (BI), a precursor for carotenoids, is widely distributed in fruit and vegetables. Recent in vitro studies have demonstrated the potential anti-metastatic effects of BI, but the mechanisms underlying such actions are not clear. Because liver cancer is the most endemic cancer in Taiwan and in a large region of the world, we hereby investigate the anti-metastatic effects of BI and its mechanisms of actions in a highly metastatic human hepatocarcinoma SK-Hep-1 cells. We show that incubation of cells with BI (1-50 mu M) for 24 and 48 h significantly inhibited cell invasion, migration and adhesion. Mechanistically, incubation of cells with BI (1-50 mu M) for 24 h resulted in the following: (1) significant inhibition of matrix metalloproteinase (MMP)-2, MMP-9 and urokinase-type plasminogen activator activities, (2) up-regulation of protein expression of the tissue inhibitor of matrix metalloproteinase (TIMP)-1, TIMP-2 and plasminogen activator inhibitor-1, (3) down-regulation of the expression of migration-related proteins, including focal adhesion kinase (FAK), phosphorylated form of FAK, Rho, Rac1 and Cdc42 and (4) up-regulation of the expression of nm23-H1 protein (P<0.05). Overall, the results show that BI effectively inhibits the metastasis of SK-Hep-1 cells, and this effect involves the regulation of gene expression and signal pathways related to invasion and migration.
URI: http://hdl.handle.net/11455/68376
ISSN: 0007-1145
文章連結: http://dx.doi.org/10.1017/s0007114511003473
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