請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/68545
標題: Salinity-dependent expression of a Na+, K+, 2Cl(-) cotransporter in gills of the brackish medaka Oryzias dancena: A molecular correlate for hyposmoregulatory endurance
作者: Kang, C.K.
Tsai, H.J.
Liu, C.C.
Lee, T.H.
Hwang, P.P.
關鍵字: Euryhaline teleost
Gill
Medaka
Na+, K+, 2Cl(-) cotransporter
Oryzias
Osmoregulation
Salinity tolerance
cation-chloride-cotransporters
mitochondria-rich cells
eel
anguilla-anguilla
acid-base regulation
cftr anion channel
salmon
salmo-salar
cl cotransporter
na+/k+/2cl(-) cotransporter
differential
expression
atlantic salmon
期刊/報告no:: Comparative Biochemistry and Physiology a-Molecular & Integrative Physiology, Volume 157, Issue 1, Page(s) 7-18.
摘要: This study used the brackish medaka (Oryzias dancena) to characterize Na+, K+, 2Cl(-) cotransporter (NKCC) expression from the genetic to cellular level in gills. Using RT-PCR to survey tissue distribution of nkcc1a, 1b. and 2, we report that gills of brackish medaka prominently express Odnkcc1a. The full-length cDNA of Odnkcc1a was cloned from gill tissue. In situ hybridization indicates that Odnkcc1a was localized to mitochondrion-rich (MR) cells. Higher mRNA levels of Odnkcc1a were found in gills from seawater (SW) and brackish water (BW) medaka when compared to freshwater (FW) fish. Furthermore, higher amounts of NKCC1a-like protein were detected by the monoclonal antibody in gills of SW and BW medaka compared to FIN medaka. Double immunofluorescence staining revealed that NKCC1a-like protein colocalizes with Na+, K+ -ATPase on the basolateral membrane of MR cells in BW and SW fish. In addition, transfer of brackish medaka from SW to FW revealed that expression of NKCC1a-like protein in gills was retained until 7 days, which is a likely mechanism for maintaining hyposmoregulatory endurance. The study illustrates salinity-dependent expression of NKCC1a in branchial MR cells from brackish medaka and suggests a critical role for NKCC1a in hyposmoregulatory endurance of this fish. (C) 2010 Elsevier Inc. All rights reserved.
URI: http://hdl.handle.net/11455/68545
ISSN: 1095-6433
文章連結: http://dx.doi.org/10.1016/j.cbpa.2010.05.013
顯示於類別:期刊論文

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