Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/68634
標題: An antifungal chitinase produced by Bacillus cereus with shrimp and crab shell powder as a carbon source
作者: Chang, W.T.
Chen, C.S.
Wang, S.L.
關鍵字: pseudomonas-aeruginosa k-187
chitinolytic enzymes
trichoderma-harzianum
circulans wl-12
purification
endochitinase
exochitinase
fluorescens
proteins
strain
期刊/報告no:: Current Microbiology, Volume 47, Issue 2, Page(s) 102-108.
摘要: The production of inexpensive chitinolytic enzymes is an element in the utilization of shellfish processing wastes. In this study, shrimp and crab shell powder prepared by treating shrimp and crab processing wastes with boiling and crushing was used as a substrate for the isolation of an antifungal chitinase-producing microorganism. Bacillus cereus YQ 308, a strain isolated from the soil samples, excreted one chitinase when cultured in a medium containing 2% (wt/vol) shrimp and crab shell powder as major carbon source. The chitinase, purified by sequential chromatography, had an Mr of 48 kDa and pI of 5.2. The purified chitinase (2 mg/ml) inhibited the hyphal extension of the fungi Fusarium oxysporum and Pythium ultimum.
URI: http://hdl.handle.net/11455/68634
ISSN: 0343-8651
文章連結: http://dx.doi.org/10.1007/s00284-002-3955-7
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