Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/68641
標題: The N-terminal signal sequence and the last 98 amino acids are not essential for the secretion of Bacillus sp TS-23 alpha-amylase in Escherichia coli
作者: Lo, H.F.
Lin, L.L.
Li, C.C.
Hsu, W.H.
Chang, C.T.
關鍵字: gram-negative bacteria
extracellular protein secretion
nucleotide-sequence
p-glycoprotein
gene
expression
alkaliphiles
determinants
peptides
cloning
期刊/報告no:: Current Microbiology, Volume 43, Issue 3, Page(s) 170-175.
摘要: A truncated Bacillus sp. TS-23 alpha -amylase gene lacking 96 and 294 bp at its 5' and 3' end respectively was prepared by polymerase chain reaction and cloned into Escherichia coli expression vector, pQE-30, under the control of T5 promoter. SDS-PAGE and activity staining analyses showed that the His(6)-tagged amylase had a molecular mass of approximately 54 kDa. Isopropyl-beta -D-thiogalactopyranoside (IPTG) induction of E. coli M15 cells bearing the recombinant plasmid resulted in the extracellular production of active amylase. Western blot analysis also revealed that the truncated amylase was present in the periplasmic space and culture medium.
URI: http://hdl.handle.net/11455/68641
ISSN: 0343-8651
文章連結: http://dx.doi.org/10.1007/s002840010282
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