Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/68904
DC FieldValueLanguage
dc.contributor.authorHuang, T.C.en_US
dc.contributor.authorHuang, H.C.en_US
dc.contributor.authorChang, C.C.en_US
dc.contributor.authorChang, H.Y.en_US
dc.contributor.authorOu, C.H.en_US
dc.contributor.authorHsu, C.H.en_US
dc.contributor.authorChen, S.T.en_US
dc.contributor.authorJuan, H.F.en_US
dc.date2007zh_TW
dc.date.accessioned2014-06-11T05:57:29Z-
dc.date.available2014-06-11T05:57:29Z-
dc.identifier.issn0014-5793zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/68904-
dc.description.abstractSynthetic peptides with the arginine-glycine-aspartate (RGD) motif have been used widely as inhibitors of integrin-ligand interactions to study cell growth, adhesion, migration and differentiation. We designed cyclic-RGD peptide (TpaRGDWPC-, cRGD) which could cause cell death in MCF-7 cell line. In order to understand the mechanism involved in cRGD-induced apoptosis, we used microarray, real-time quantitative PCR (Q-PCR) and bioinformatics to study the effects of cRGD on breast cancer cell line MCF-7. By time-series gene expression measurements and our developed software BSIP and GeneNetwork, we reconstructed an apoptosis-related gene network. In the network, caspase-9, located at the upstream, activates downstream effector caspases such as caspase-7, leading to the induction of caspase-4. Combined our previous proteomics data with newly performed docking simulation, it indicated that the cell death induced by cRGD may be triggered through blocking integrin signaling to the extracellular matrix and activation of caspase pathway. This study provides a molecular explanation of cRGD treatment in breast cancer cells and set forth a constructive far-reaching impact on breast cancer therapy. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.en_US
dc.language.isoen_USzh_TW
dc.relationFebs Lettersen_US
dc.relation.ispartofseriesFebs Letters, Volume 581, Issue 18, Page(s) 3517-3522.en_US
dc.relation.urihttp://dx.doi.org/10.1016/j.febslet.2007.06.067en_US
dc.subjectapoptosisen_US
dc.subjectcRGDen_US
dc.subjectintegrinen_US
dc.subjectMCF-7en_US
dc.subjectcaspaseen_US
dc.subjectrgd-peptidesen_US
dc.subjectcaspase-3 activationen_US
dc.subjectantagonistsen_US
dc.subjectexpressionen_US
dc.subjectligandsen_US
dc.subjectdockingen_US
dc.subjectcomplexen_US
dc.subjectdeathen_US
dc.titleAn apoptosis-related gene network induced by novel compound-cRGD in human breast cancer cellsen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1016/j.febslet.2007.06.067zh_TW
Appears in Collections:期刊論文
文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.