Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/68928
標題: Simultaneous' flow cytometric assessment for cellular types and phagocytic abilities of the haemocytes of the hard clam, Meretrix lusoria
作者: Tu, C.Y.
Hung, S.W.
Tsou, L.T.
Chang, Y.C.
Wang, W.S.
關鍵字: flow cytometry
clam
Meretrix lusoria
haemocytes
phagocytosis
cell
type
Vibrio vulnificus
mytilus-galloprovincialis hemocytes
density gradient centrifugation
decussatus mollusca-bivalvia
european flat oyster
crassostrea-virginica
nitric-oxide
pathogenic vibrios
oxidative
burst
eastern oysters
pecten-maximus
期刊/報告no:: Fish & Shellfish Immunology, Volume 23, Issue 1, Page(s) 16-23.
摘要: The aim of this study was to devise a simple protocol for flow cytometric analysis to separate various haemocytic populations of the hard clam Meretrix lusoria based on the mitochondrial membrane potential diversity detected by the fluorescence probe 3,3-dihexyloxacarbocyanine iodide (DiOC6). Compared with the traditional technique for separation of haemocytic populations, continuous Percoll gradient centrifugation, our novel method was more efficient and yielded a higher ratio in separating the clams' haemocytic populations. Based on fluorescence I (FL-1) and side scatter (SSC) analysis for haemocytes stained with various fluorescent densities of DiOC6 using flow cytometer, the data showed that there were three obvious cell regions R1, R2, and R3, identified by hyalinocytes, small granulocytes and large granulocytes, respectively. At the same time our results showed that the percentages of haemocytes in R1, R2, and R3 were 49.71 +/- 0.65%., 19.35 +/- 00.74%, 30.94 +/- 0.69%, respectively. After classifying the haemocytic populations, phagocytic activity of the haemocytes was simultaneously analysed with phycoerythrin (PE)-labeled Vibrio vulnificus and detected by flow cytometry. Our results demonstrated that there were higher percentages of large granulocytes compared with hyalinocytes and the percentage of small granulocytes was related to the mitochondrial membrane potential and phagocytic activities. (c) 2006 Elsevier Ltd. All rights reserved.
URI: http://hdl.handle.net/11455/68928
ISSN: 1050-4648
文章連結: http://dx.doi.org/10.1016/j.fsi.2006.08.021
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