Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/69300
標題: Application of bubble separation for quantitative analysis of choline in Dioscorea (Yam) tubers
作者: Fu, Y.C.
Chen, S.H.
Huang, P.Y.
Li, Y.J.
關鍵字: choline
Dioscorea
yam
centrifugation
mucilage
bubble separation
期刊/報告no:: Journal of Agricultural and Food Chemistry, Volume 53, Issue 7, Page(s) 2392-2398.
摘要: A modified assay based on the AACC official method 86-45 (AACC, 2000) for the determination of choline in three cereals and three varieties of Dioscorea (yam) tubers was developed. When tested in wheat, rice, and oat flour, choline estimated by the modified method was 34.0-45.3% higher than that of the original AACC method. In a system with higher contents of starch and mucilage, such as Dioscorea (yam) tubers, extra procedures in sample preparation needed to be carried out to separate starch and mucilage. The choline contents of the following Dioscorea (yam) tubers using the original AACC method and the present modified AACC method through coupling an additional bubble separation procedure, respectively, were (mean +/- SD, mg/g solid) Keelung yam (D. pseudojaponica Y.) 0.92 +/- 0.09 and 2.21 +/- 0.12, Yangmingshan yam (D. alata L.) 0.77 +/- 0.09 and 1.78 +/- 0.28, and Ming-Chien yam (D. purpurea) 0.44 +/- 0.09 and 1.35 +/- 0.19. Choline was 231-306% higher than when the original AACC method was used. Dioscorea (yam) tubers were much higher in choline content than they were in cereals. Bubble separation is an appropriate procedure in the practice for the maximum assay of choline in yams. It is accurate, rapid, easy to handle, and especially good for recovering choline from a starch and polysaccharide-protein-containing system.
URI: http://hdl.handle.net/11455/69300
ISSN: 0021-8561
文章連結: http://dx.doi.org/10.1021/jf048501h
Appears in Collections:期刊論文

文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.