Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/69380
標題: Sequencing of an internal transcribed spacer region of 16S-23S rRNA gene and designing of PCR primers for the detection of Salmonella spp. in food
作者: Chiu, T.H.
Chen, T.R.
Hwang, W.Z.
Tsen, H.Y.
關鍵字: Salmonella
ITS
PCR
escherichia-coli
intergenic spacer
identification
probes
deletion
operon
期刊/報告no:: International Journal of Food Microbiology, Volume 97, Issue 3, Page(s) 259-265.
摘要: DNA sequences of an internal transcribed spacer (ITS) region for 40 Salmonella serovars were determined and compared with ITS sequences of Salmonella spp., and non-Salmonella spp. already available on the GenBank database. From such comparison, two Salmonella-specific ITS based PCR primers, ITSF and ITSR, were designed. When Salmonella strains with various serotypes were PCR assayed with primers ITSF/ITSR, all generated PCR products with molecular weight bands equal to 312 bp. On the other hand, 48 non-Salmonella isolates, including strains of Enterobacteriaceae and other food pathogens generated negative results. Detection limits of this PCR method was 1-9 CFU per assay. These PCR primers were used for the detection of Salmonella cells in artificially contaminated foods, including chicken meat and whole milk. The detection limit was 1-9 X 10(3) CFU per assay. With an 8-h enrichment step performed prior to the PCR assay, however, the detection limit became 1-9 CFU per gram of the food sample. (C) 2004 Published by Elsevier B.V.
URI: http://hdl.handle.net/11455/69380
ISSN: 0168-1605
文章連結: http://dx.doi.org/10.1016/j.ijfoodmicro.2004.04.005
Appears in Collections:期刊論文

文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.