Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/69409
標題: Efficient hydrolysis of tuna oil by a surfactant-coated lipase in a two-phase system
作者: Ko, W.C.
Wang, H.J.
Hwang, J.S.
Hsieh, C.W.
關鍵字: tuna oil
surfactant-coated lipase
Candida rugosa
polyunsaturated
fatty acid
kinetics
polyunsaturated fatty-acids
candida-cylindracea lipase
docosahexaenoic
acid
fish-oil
selective esterification
eicosapentaenoic acid
organic-solvents
rugosa lipase
enrichment
triglyceride
期刊/報告no:: Journal of Agricultural and Food Chemistry, Volume 54, Issue 5, Page(s) 1849-1853.
摘要: A surfactant-coated lipase (SCL) prepared by mixing Candida rugosa lipase with emulsifier in ethanol was used to hydrolyze tuna oil in a two-phase aqueous-organic system. Both enzyme (SCL) and substrate (tuna oil) were soluble in the organic phase, and the hydrolysis could occur with water molecules from the aqueous phase. This hydrolysis could promptly proceed compared to that catalyzed by native lipases which only occurred at the interface between the two phases. Michaelis-Menten kinetics in the two-phase reactions showed that the Km value of the SCL was half that of the native lipase, while the maximum velocity V-max was 11.5 times higher. The hydrolysis method resulted in enrichment of n-3 polyunsaturated fatty acid (n-3 PUFA) content in glyceride mixtures from 26.4% to 49.8% and DHA from 19.1% to 38.9%. The SCL acted as an efficient hydrolytic catalyst for tuna oil.
URI: http://hdl.handle.net/11455/69409
ISSN: 0021-8561
文章連結: http://dx.doi.org/10.1021/jf051937s
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