Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/69436
標題: Molecular, monitoring of microbes in a continuous hydrogen-producing system with different hydraulic retention time
作者: Chang, J.J.
Wu, J.H.
Wen, F.S.
Hung, K.Y.
Chen, Y.T.
Hsiao, C.L.
Lin, C.Y.
Huang, C.C.
關鍵字: bio-hydrogen
clostridium
condensed molasses fermentation
soluble
DGGE
hydrogenase
RT-PCR
waste-water
clostridium-acetobutylicum
anaerobic-bacteria
fermentation
glucose
sludge
microflora
cultures
reactor
期刊/報告no:: International Journal of Hydrogen Energy, Volume 33, Issue 5, Page(s) 1579-1585.
摘要: An anaerobic continuous-flow fermentation system with condensed molasses fermentation soluble (CMS) from monosodium glutamate production as substrate was developed for the biohydrogen production. The production rate of the system was increased, while hydraulic retention time (HRT) was varied from 12 to 3 h and the optimal hydrogen production rate was obtained at HRT 3 h. The major bacterial composition of each HRT was analyzed by 16S rRNA gene-targeted denaturing gradient gel electrophoresis (DGGE). Acidaminococcus sp. that could evolve hydrogen from glutamate was the only hydrogen producer and could be also detected from substrate. Hydrogenase mRNA-targeted reverse transcriptase polymerase chain reaction (RT-PCR) analysis showed that except HRT 3 h, two clusters of clostridial hydrogenase gene sequences were obtained. RT-PCR-DGGE analysis was employed to illustrate the active clostridial strains confirming that clostridial strains were not present at HRT 3 h. Acidaminococcus sp. existing in substrate might be the major strain that contributes to hydrogen production at HRT 3 h. (c) 2007 International Association for Hydrogen Energy. Published by Elsevier Ltd. All rights reserved.
URI: http://hdl.handle.net/11455/69436
ISSN: 0360-3199
文章連結: http://dx.doi.org/10.1016/j.ijhydene.2007.09.04S
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