Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/69495
標題: Zanthoxylum avicennae extracts inhibit cell proliferation through protein phosphatase 2A activation in HA22T human hepatocellular carcinoma cells in vitro and in vivo
作者: Dung, T.D.
Chang, H.C.
Binh, T.V.
Lee, M.R.
Tsai, C.H.
Tsai, F.J.
Kuo, W.W.
Chen, L.M.
Huang, C.Y.
關鍵字: hepatocellular carcinoma
Zanthoxylum avicennae (Ying Bu Bo)
protein
phosphatase 2A
cell proliferation
nude mouse model
p53 ubiquitination
apoptosis
mdm2
mice
epidemiology
pathogenesis
growth
gene
期刊/報告no:: International Journal of Molecular Medicine, Volume 29, Issue 6, Page(s) 1045-1052.
摘要: Hepatocellular carcinoma is a common type of cancer that is usually associated with poor prognosis. In this study, we examined the in vitro and in vivo mechanisms of the traditional Vietnamese herb Zanthoxylum avicennae on the inhibition of HA22T human hepatocellular carcinoma cell proliferation. HA22T cells were treated with different concentrations of Zanthoxylum avicennae extracts (YBBEs) and analyzed with the MTT assay, western blot analysis, flow cytometry, siRNA transfection assays and co-immunoprecipitation assay. Additionally, the HA22T-implanted xenograft nude mouse model was applied to confirm the cellular effects. YBBEs showed a strong inhibition of HA22T cell viability in a dose-dependent manner and significantly reduced cell proliferation-related proteins as well as induced cell cycle arrest in the G2/M phase. Protein phosphatase 2A (PP2A) siRNA or okadaic acid totally blocked YBBE-mediated cell proliferation inhibition. In addition, an HA22T-implanted nude mouse model further confirmed that YBBEs inhibit HA22T tumor cell growth and downregulate the survival and cell cycle regulating proteins, as well as activate the PP2A protein. Our findings indicate that the inhibition of HA22T cell proliferation by YBBEs is mediated through PP2A activation.
URI: http://hdl.handle.net/11455/69495
ISSN: 1107-3756
文章連結: http://dx.doi.org/10.3892/ijmm.2012.938
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