Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/69540
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dc.contributor.authorChou, J.H.en_US
dc.contributor.authorJiang, S.R.en_US
dc.contributor.authorCho, J.C.en_US
dc.contributor.authorSong, J.en_US
dc.contributor.authorLin, M.C.en_US
dc.contributor.authorChen, W.M.en_US
dc.date2008zh_TW
dc.date.accessioned2014-06-11T05:58:28Z-
dc.date.available2014-06-11T05:58:28Z-
dc.identifier.issn1466-5026zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/69540-
dc.description.abstractThree Gram-negative, non-pigmented, rod-shaped, facultatively aerobic bacterial strains, designated d8-1(T), d8-2 and IMCC1716, were isolated from a freshwater spring sample and a eutrophic freshwater pond. Based on characterization using a polyphasic approach, the three strains showed highly similar phenotypic, physiological and genetic characteristics. All of the strains harboured the nitrogenase gene nifH, but nitrogen-fixing activities could not be detected in nitrogen-free culture media. The three strains shared 99.6-99.7 % 16S rRNA gene sequence similarity and showed 89-100 % DNA-DNA relatedness, suggesting that they represent a single genomic species. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains d8-1(T), d8-2 and IMCC1716 formed a monophyletic branch in the periphery of the evolutionary radiation occupied by the genus Azonexus. Their closest neighbours; were Azonexus caeni Slu-05(T) (96.7-96.8% similarity) and Azonexus fungiphilus BS5-8(T) (96.3-96.6 %). The DNA-DNA relatedness of the novel strains to these two species of the genus Azonexus was less than 70%. The isolates could also be differentiated from recognized members of the genus Azonexus on the basis of phenotypic and biochemical characteristics. It is evident, therefore, that the three strains represent a novel species of the genus Azonexus, for which the name Azonexus hydrophilus sp. nov. is proposed. The type strain is d8-1(T) (=LMG 24005(T)=BCRC 17657 (T)).en_US
dc.language.isoen_USzh_TW
dc.relationInternational Journal of Systematic and Evolutionary Microbiologyen_US
dc.relation.ispartofseriesInternational Journal of Systematic and Evolutionary Microbiology, Volume 58, Page(s) 946-951.en_US
dc.relation.urihttp://dx.doi.org/10.1099/ijs.0.65434-0en_US
dc.subjectdeoxyribonucleic-aciden_US
dc.subjectpcren_US
dc.subjectproteobacteriaen_US
dc.subjectidentificationen_US
dc.subjectsequencesen_US
dc.subjectstrainsen_US
dc.titleAzonexus hydrophilus sp nov., a nifH gene-harbouring bacterium isolated from freshwateren_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1099/ijs.0.65434-0zh_TW
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