Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/69688
標題: Development and use of 16S rRNA gene targeted PCR primers for the identification of Escherichia coli cells in water
作者: Tsen, H.Y.
Lin, C.K.
Chi, W.R.
關鍵字: polymerase chain-reaction
ribosomal-rna
enzymatic amplification
dna
hybridization
toxin-i
probes
salmonella
foods
oligonucleotides
bacteria
期刊/報告no:: Journal of Applied Microbiology, Volume 85, Issue 3, Page(s) 554-560.
摘要: The primary sequences of the V-3 and V-6 regions of the 16S rRNA gene of pathogenic and non-pathogenic strains of Escherichia coli were determined and compared with those obtained for a number of reference strains which belong to the family Enterobacteriaceae. Three oligonucleotide primers 16E1, 16E2, and 16E3 were designed and used in the polymerase chain reaction to identify specifically all E. coli isolates. When 16E1, 16E2 ann 16E3 were used as primers for the identification of E. coli cells present in tap, underground and pond waters, as low as I cfu 100 ml(-1) of water could be detected if an a h pre-culture step was performed prior to the PCR reaction.
URI: http://hdl.handle.net/11455/69688
ISSN: 1364-5072
文章連結: http://dx.doi.org/10.1046/j.1365-2672.1998.853535.x
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