請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/69764
標題: Comparison between two different methods of immobilizing NGF in poly(DL-lactic acid-co-glycolic acid) conduit for peripheral nerve regeneration by EDC/NHS/MES and genipin
作者: Hsieh, S.C.
Tang, C.M.
Huang, W.T.
Hsieh, L.L.
Lu, C.M.
Chang, C.J.
Hsu, S.H.
關鍵字: chitosan
nerve growth factor
poly (DL-lactic acid-co-glycolic acid)
peripheral nerve regeneration
growth-factor
guidance channels
polyglycolic acid
in-vitro
chitosan
release
reconstruction
migration
heparin
surface
期刊/報告no:: Journal of Biomedical Materials Research Part A, Volume 99A, Issue 4, Page(s) 576-585.
摘要: For surface modification and nerve regeneration, chitosan, followed by nerve growth factor (NGF), was immobilized onto the interior surface of poly (lactic acit-co-glycolic) conduits, using EDC/NHS/MES system (EDCs) and genipin (GP). Four new conduits were, therefore, obtained and named by immobilizing order-EDCs/EDCs, GP/EDCs, EDCs/GP, and GP/GP groups. The immobilized methods used were evaluated and compared, respectively. The researchers found that the EDCs- and GP-cross-linked chitosan displayed higher hydrophilic than pure poly (DL-lactic acid-co-glycolic acid) (PLGA) in water contact angle experiment, which meant the cell compatibility was improved by the modification. Scanning electron microscopic observations revealed that the GP-cross-linking of chitosan greatly improved cell compatibility while cultured rat PC12 cells were flatter and more spindle-shaped than EDCs-cross-linked chitosan. The results concerning the GP-crosslinked chitosan revealed significant proliferation of the seeded cells relative to pure PLGA films, as determined by counting cells and MTT assay. The NGF was released from the modified conduits in two separate periods-an initial burst in 5 days and then slow release from day 10 to day 40. The GP/EDCs group had the highest NGF value among all groups after the 5th day. Finally, the controlled-release conduits were used to bridge a 10 mm rat sciatic nerve defect. Six weeks following implantation, morphological analysis revealed the highest numbers of myelinated axons in the midconduit and distal regenerated nerve in GP/EDCs group. Therefore, the results confirm that GP/EDCs groups with good cell compatibility and effective release of NGF can considerably improve peripheral nerve regeneration. (C) 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 99A: 576-585, 2011.
URI: http://hdl.handle.net/11455/69764
ISSN: 1549-3296
文章連結: http://dx.doi.org/10.1002/jbm.a.33157
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