Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/69779
標題: Effect of membranes with various hydrophobic/hydrophilic properties on lipase immobilized activity and stability
作者: Chen, G.J.
Kuo, C.H.
Chen, C.I.
Yu, C.C.
Shieh, C.J.
Liu, Y.C.
關鍵字: Lipase
Covalent immobilization
Polyvinylidene fluoride
Hydrophobicity
Surface modification
metal affinity membranes
covalent immobilization
candida-rugosa
purification
chitosan
chromatography
enhancement
hydrolysis
adsorption
期刊/報告no:: Journal of Bioscience and Bioengineering, Volume 113, Issue 2, Page(s) 166-172.
摘要: In this study, three membranes: regenerated cellulose (RC), glass fiber (GF) and polyvinylidene fluoride (PVDF), were grafted with 1,4-diaminobutane (DA) and activated with glutaraldehyde (GA) for lipase covalent immobilization. The efficiencies of lipases immobilized on these membranes with different hydrophobic/hydrophilic properties were compared. The lipase immobilized on hydrophobic PVDF-DA-GA membrane exhibited more than an 11-fold increase in activity compared to its immobilization on a hydrophilic RC-DA-GA membrane. The relationship between surface hydrophobicity and immobilized efficiencies was investigated using hydrophobic/hydrophilic GF membranes which were prepared by grafting a different ratio of n-butylamine/1,4-diaminobutane (BA/DA). The immobilized lipase activity on the GF membrane increased with the increased BA/DA ratio. This means that lipase activity was exhibited more on the hydrophobic surface. Moreover, the modified PVDF-DA membrane was grafted with GA, epichlorohydrin (EPI) and cyanuric chloride (CC), respectively. The lipase immobilized on the PVDF-DA-EPI membrane displayed the highest specific activity compared to other membranes. This immobilized lipase exhibited more significant stability on pH, thermal, reuse, and storage than did the free enzyme. The results exhibited that the EPI modified PVDF is a promising support for lipase immobilization. (C) 2011, The Society for Biotechnology, Japan. All rights reserved.
URI: http://hdl.handle.net/11455/69779
ISSN: 1389-1723
文章連結: http://dx.doi.org/10.1016/j.jbiosc.2011.09.023
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