請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/69812
標題: Determination of Alachlor and Its Metabolite 2,6-Diethylaniline in Microbial Culture Medium Using Online Microdialysis Enriched-Sampling Coupled to High-Performance Liquid Chromatography
作者: Chen, C.Z.
黃振文
Yan, C.T.
Kumar, P.V.
Huang, J.W.
Jen, J.F.
鄭政峰
關鍵字: alachlor
2,6-diethylaniline (2,6-DEA)
hollow fiber
liquid phase
microextraction
HPLC-UV
microbial culture medium
solid-phase microextraction
ionization mass-spectrometry
priority
pesticides
extraction
water
gas
degradation
herbicides
hplc
products
期刊/報告no:: Journal of Agricultural and Food Chemistry, Volume 59, Issue 15, Page(s) 8078-8085.
摘要: In this study, a simple and novel microdialysis sampling technique incorporating hollow fiber liquid phase microextraction (HF-LPME) coupled online to high-performance liquid chromatography (HPLC) for the one-step sample pretreatment and direct determination of alachlor (2-chloro-2',6'-diethyl-N -(methoxymethyl)acetanilide) and its metabolite 2,6-diethylaniline (2,6-DEA) in microbial culture medium has been developed. A reversed-phase C-18 column was utilized to separate alachlor and 2,6-DEA from other species using an acetonitrile/water mixture (1:1) containing 0.1 M phosphate buffer solution at pH 7.0 as the mobile phase. Detection was carried out with a UV detector operated at 210 nm. Parameters that influenced the enrichment efficiency of online HF-LPME sampling, including the length of the hollow fiber, the perfusion solvent and its flow rate, the pH, and the salt added in sample solution, as well as chromatographic conditions were thoroughly optimized. Under optimal conditions, excellent enrichment efficiency was achieved by the microdialysis of a sample solution (pH 7.0) using hexane as perfusate at the flow rate of 4 mu L/min. Detection limits were 72 and 14 ng/mL for alachlor and 2,6-DEA, respectively. The enrichment factors were 403 and 386 (RSD < 5%) for alachlor and 2,6-DEA, respectively, when extraction was performed by using a 40 cm regenerated cellulose hollow fiber and hexane as perfusion solvent at the flow rate of 0.1 mu L/min. The proposed method provides a sensitive, flexible, fast, and eco-friendly procedure to enrich and determine alachlor and its metabolite (2,6-DEA) in microbial culture medium.
URI: http://hdl.handle.net/11455/69812
ISSN: 0021-8561
文章連結: http://dx.doi.org/10.1021/jf201129j
顯示於類別:農業暨自然資源學院

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