Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/69831
標題: Determination of catecholamines in pheochromocytoma cell (PC-12) culture medium by microdialysis-microbore liquid chromatography
作者: Cheng, F.C.
Kuo, J.S.
Huang, H.M.
Yang, D.Y.
Wu, T.F.
Tsai, T.H.
關鍵字: pheochromocytoma cells
catecholamine
nerve growth-factor
electrochemical detection
corpus-luteum
pc12
cells
serotonin
dopamine
release
metabolites
inhibition
oxytocin
期刊/報告no:: Journal of Chromatography A, Volume 870, Issue 1-2, Page(s) 405-411.
摘要: An in vitro microdialysis system was constructed for the measurement of catecholamines in pheochromocytoma cell culture medium. The novel microdialysis device is composed of a petri dish, a dialysis membrane and two transmission tubes. The dialysis membrane is located in the space of a petri dish such that it is immersed in the culture medium. Catecholamines contained in the culture medium diffused into a designed dialysis membrane with sufficient recovery (about 60%). Dialysates were collected by a sampling loop and introduced by an on-line injector to a microbore liquid chromatographic system for analysis of catecholamines. This assay yielded a detection Limit of 0.2-0.5 pg/injection with acceptable intra- and inter-assay reproducibilities in 5 mu l of dialysates. To evaluate the on-line microdialysis system, PC-12 cells were cultured in a petri dish within an incubator. The baseline concentration of dopamine in PC-12 cell culture medium was about 0.29 ng/ml which was elevated to 2.43 ng/ml after treatment with 0.5 mM potassium cyanide. In conclusion, the present microassay provides for the sensitive, direct measurement of catecholamines in culture medium while minimizing pretreatment procedures for sample preparation. (C) 2000 Elsevier Science B.V. All rights reserved.
URI: http://hdl.handle.net/11455/69831
ISSN: 0021-9673
文章連結: http://dx.doi.org/10.1016/s0021-9673(99)00910-3
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