請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/69837
標題: Inhibition of soil-borne plant pathogens by the treatment of sinigrin and myrosinases released from reconstructed Escherichia coli and Pichia pastoris
作者: Chung, W.C.
黃振文
Huang, H.C.
Chiang, B.T.
Huang, J.W.
關鍵字: Escherichia coli
Pichia pastoris
myrosinase genes TGG1 and TGG2
Arabidopsis thaliana
sinigrin
allyl isothiocyanate
Rhizoctonia solani
brassica-napus
gene family
arabidopsis-thaliana
expression
organization
evolution
products
seed
期刊/報告no:: Biocontrol Science and Technology, Volume 15, Issue 5, Page(s) 455-465.
摘要: Myrosinases ( thioglucoside glucohydrolase, EC 3.2.3.1) are able to hydrolyse glucosinolates in natural plant products. In Arabidopsis thaliana three different genes with different tissue- specific expressions and distribution patterns encode myrosinases. cDNAs of myrosinase genes ( TGG1 and TGG2) were isolated from A. thaliana and expressed in Escherichia coli and Pichia pastoris. The enzyme activities of myrosinase TGG1 and TGG2 genes expressed in P. pastoris were higher than those expressed in E. coli. Among six glucosinolates tested for specificity to myrosinases TGG1 and TGG2, the suitable substrates for these two genes expressed in P. pastoris and E. coli were sinigrin, gluconapin, glucobrassicanapin and glucoraphanin. Treatment of sinigrin with myrosinases excreted from reconstructed E. coli and P. pastoris with TGG1 and TGG2 genes showed strong fungicidal effects on mycelial growth of Rhizoctonia solani AG- 4, Sclerotium rolfsii, and Pythium aphanidermatum. This study suggests that the combination of glucosinolate with myrosinases excreted from the reconstructed microbes may be of potential for control of soil- borne diseases.
URI: http://hdl.handle.net/11455/69837
ISSN: 0958-3157
文章連結: http://dx.doi.org/10.1080/09583150500086607
顯示於類別:農業暨自然資源學院

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