請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/69842
標題: The ability of LCRMP-1 to promote cancer invasion by enhancing filopodia formation is antagonized by CRMP-1
作者: Pan, S.H.
Chao, Y.C.
Hung, P.F.
Chen, H.Y.
Yang, S.C.
Chang, Y.L.
Wu, C.T.
Chang, C.C.
Wang, W.L.
Chan, W.K.
Wu, Y.Y.
Che, T.F.
Wang, L.K.
Lin, C.Y.
Lee, Y.C.
Kuo, M.L.
Lee, C.H.
Chen, J.J.W.
Hong, T.M.
Yang, P.C.
關鍵字: response mediator protein-1
aldrich-syndrome protein
wave family
proteins
growth cone collapse
actin-based motility
n-wasp
arp2/3
complex
optical microscopy
shigella-flexneri
lung-cancer
期刊/報告no:: Journal of Clinical Investigation, Volume 121, Issue 8, Page(s) 3189-3205.
摘要: Metastasis is a predominant cause of death in patients with cancer. It is a complex multistep process that needs to be better understood if we are to develop new approaches to managing tumor metastasis. Tumor cell invasion of the local stroma is suppressed by collapsin response mediator protein-1 (CRMP-1). Recently, we identified a long isoform of CRMP-1 (LCRMP-1), expression of which correlates with cancer cell invasiveness and poor clinical outcome in patients with non-small-cell lung cancer (NSCLC). Here, we report that LCRMP-1 overexpression in noninvasive human cell lines enhanced filopodia formation, cancer cell migration, and invasion via stabilization of actin. This effect required a highly conserved N-terminal region of LCRMP-1 as well as the WASP family verprolin-homologous protein-1/actin nucleation pathway (WAVE-1/actin nucleation pathway). Furthermore, LCRMP-1 appeared to act downstream of Cdc42, a Rho family protein known to be involved in actin rearrangement. In addition, LCRMP-1 associated with CRMP-1, which downregulated cancer cell metastasis by interrupting the association of LCRMP-1 and WAVE-1. Finally, we found that high-level expression of LCRMP-1 and low-level expression of CRMP-1 were associated with lymph node metastasis and poor survival in patients with NSCLC. In sum, we show that LCRMP-1 and CRMP-1 have opposing functions in regulating cancer cell invasion and metastasis and propose that this pathway may serve as a potential anticancer target.
URI: http://hdl.handle.net/11455/69842
ISSN: 0021-9738
文章連結: http://dx.doi.org/10.1172/jci42975
顯示於類別:期刊論文

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