Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/69857
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dc.contributor.authorYang, W.K.en_US
dc.contributor.authorKang, C.K.en_US
dc.contributor.authorChen, T.Y.en_US
dc.contributor.authorChang, W.B.en_US
dc.contributor.authorLee, T.H.en_US
dc.date2011zh_TW
dc.date.accessioned2014-06-11T05:59:01Z-
dc.date.available2014-06-11T05:59:01Z-
dc.identifier.issn0174-1578zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/69857-
dc.description.abstractIn the branchial mitochondrion-rich (MR) cells of euryhaline teleosts, the Na+/K+/2Cl(-)cotransporter (NKCC) is an important membrane protein that maintains the internal Cl -concentration, and the branchial Na+/K+-ATPase (NKA) is crucial for providing the driving force for many other ion-transporting systems. Hence this study used the sailfin molly (Poecilia latipinna), an introduced aquarium fish in Taiwan, to reveal that the potential roles of NKCC and NKA in sailfin molly were correlated to fish survival rates upon salinity challenge. Higher levels of branchial NKCC were found in seawater (SW)-acclimated sailfin molly compared to freshwater (FW)-acclimated individuals. Transfer of the sailfin molly from SW to FW revealed that the expression of the NKCC and NKA proteins in the gills was retained over 7 days in order to maintain hypoosmoregulatory endurance. Meanwhile, their survival rates after transfer to SW varied with the duration of FW-exposure and decreased significantly when the SW-acclimated individuals were acclimated to FW for 21 days. Double immunofluorescence staining showed that in SW-acclimated sailfin molly, NKCC signals were expressed on the basolateral membrane of MR cells, whereas in FW-acclimated molly, they were expressed on the apical membrane. This study illustrated the correlation between the gradual reductions in expression of branchial NKCC and NKA (i.e., the hypoosmoregulatory endurance) and decreasing survival rates after hyperosmotic challenge in sailfin molly.en_US
dc.language.isoen_USzh_TW
dc.relationJournal of Comparative Physiology B-Biochemical Systemic and Environmental Physiologyen_US
dc.relation.ispartofseriesJournal of Comparative Physiology B-Biochemical Systemic and Environmental Physiology, Volume 181, Issue 7, Page(s) 953-964.en_US
dc.relation.urihttp://dx.doi.org/10.1007/s00360-011-0568-0en_US
dc.subjectSalinity toleranceen_US
dc.subjectNa+/K+/2Cl(-) cotransporteren_US
dc.subjectNa+/K+-ATPaseen_US
dc.subjectGillen_US
dc.subjectOsmoregulationen_US
dc.subjectSailfin mollyen_US
dc.subjecttilapia oreochromis-mossambicusen_US
dc.subjectmitochondrion-rich cellsen_US
dc.subjectcation-chloride-cotransportersen_US
dc.subjecteel anguilla-anguillaen_US
dc.subjectfresh-wateren_US
dc.subjectpoecilia-latipinnaen_US
dc.subjectmessenger-rnaen_US
dc.subjectdifferential expressionen_US
dc.subjectmolecularen_US
dc.subjectphysiologyen_US
dc.subjecteuryhaline tilapiaen_US
dc.titleSalinity-dependent expression of the branchial Na+/K+/2Cl(-) cotransporter and Na+/K+-ATPase in the sailfin molly correlates with hypoosmoregulatory enduranceen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1007/s00360-011-0568-0zh_TW
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