請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/69863
標題: Development of the molecular methods for rapid detection and differentiation of Fusarium oxysporum and F. oxysporum f. sp niveum in Taiwan
作者: Lin, Y.H.
黃振文
Chen, K.S.
Chang, J.Y.
Wan, Y.L.
Hsu, C.C.
Huang, J.W.
Chang, P.F.L.
關鍵字: formae-speciales
pcr assays
race
wilt
resistance
watermelon
culmorum
gene
graminearum
avenaceum
期刊/報告no:: New Biotechnology, Volume 27, Issue 4, Page(s) 409-418.
摘要: Fusarium wilt, caused by Fusarium oxysporum (Fo), is one of the most important fungal diseases worldwide. Like other plant pathogens, Fo displays specialized forms in association with its hosts. For example, F. oxysporum f. sp. niveum (Fon) is the damaging pathogen causing Fusarium wilt disease on watermelon, whereas F. oxysporum f. sp. cubense is the pathogen that infects banana. A rapid and reliable pathogen identification or disease diagnosis is essential for the integrated disease management practices in many crops. In this study, two new primer sets, Fon-1/Fon-2 and FnSc-1/FnSc-2, were developed to differentiate Fon and Fo, respectively. The PCR method using the novel primer sets has high sensitivity to detect Fon when the DNA concentration was as low as 0.01 pg or when the conidia number was as few as 5. In comparison with the published primer set, the Fon-1/Fon-2 primer set, derived from the sequence of OP-M12 random primer-amplified fragment, produced a 174 bp DNA fragment, and was more specific to Fon in Taiwan. In addition, with optimized PCR parameters, the molecular method using the Fon-1/Fon-2 primer set could directly detect Fon even when watermelon samples were collected in its early stage of disease development.
URI: http://hdl.handle.net/11455/69863
ISSN: 1871-6784
顯示於類別:農業暨自然資源學院

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