Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/69968
標題: Development of PCR primers for the detection of Salmonella enterica serovar Choleraesuis based on the fliC gene
作者: Chiu, T.H.
Pang, J.C.
Hwang, W.Z.
Tsen, H.Y.
關鍵字: polymerase-chain-reaction
multiplex pcr
samples
identification
enrichment
pigs
typhimurium
antigens
humans
typhi
期刊/報告no:: Journal of Food Protection, Volume 68, Issue 8, Page(s) 1575-1580.
摘要: Salmonella enterica serovar Choleraesuis may cause swine salmonellosis and human infection. Because the conventional method for detection of this Salmonella serovar may take 3 to 5 days, a PCR method for detection was evaluated. By comparing the sequence of the phase 1 flagellin (fliC) gene of Salmonella Choleraesuis with that of other Salmonella serovars and of other bacteria species available in GenBank, two PCR primers (flinC-F and flinC-R) were designed. Using these primers, all 97 Salmonella Choleraesuis strains assayed generated the expected PCR product, with a molecular mass of 963 bp. Except for S. enterica Paratyphi C, Salmonella isolates other than Salmonella Choleraesuis and non-Salmonella isolates, including strains of Enterobacteriaceae, all generated negative PCR results. Salmonella Paratyphi C could be differentiated from Salmonella Choleraesuis through the use of primers designed from the viaB gene. When Salmonella Choleraesuis isolates from swine stool, pork, liver, feed, and human whole blood samples were assayed with a preenrichment step, as low as 1 CFU/g or ml of the original sample could be detected.
URI: http://hdl.handle.net/11455/69968
ISSN: 0362-028X
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