Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/69989
標題: Subgenomic RNAs of bamboo mosaic potexvirus-V isolate are packaged into virions
作者: Lee, Y.S.
Lin, B.Y.
Hsu, Y.H.
Chang, B.Y.
Lin, N.S.
關鍵字: complete nucleotide-sequence
potato-virus-x
coat protein
messenger-rna
sindbis virus
satellite rna
genomic rna
initiation
region
identification
期刊/報告no:: Journal of General Virology, Volume 79, Page(s) 1825-1832.
摘要: Purified virions of bamboo mosaic potexvirus-V isolate (BaMV-V) were found to contain three major RNA species, the 6.4 kb genomic RNA and two RNAs of 2.0 and 1.0 kb, in addition to associated satellite RNA (0.85 kb). Results of Northern blot hybridization, primer extension analysis and cDNA sequencing showed that the packaged 2.0 and 1.0 kb RNAs of BaMV-V were subgenomic RNAs. In contrast, in the BaMV-O isolate, only genomic RNA was packaged and encapsidated subgenomic RNAs were not detectable. The transcription initiation sites for the 2.0 and 1.0 kb subgenomic RNAs of BaMV-V were located 11 and 16 nt upstream of the initiation codon of open reading frames (ORFs) 2 and 5, respectively. The 2.0 and 1.0 kb subgenomic RNAs functioned as messengers for the ORF2 protein and capsid protein, respectively. Packaging of the 1.0 kb subgenomic RNAs resulted in the formation of rod-shaped particles about 70 nm in length. Our results indicate that BaMV isolates have evolved distinctly for packaging of subgenomic RNAs.
URI: http://hdl.handle.net/11455/69989
ISSN: 0022-1317
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