Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/70110
標題: Changes in the morphology and cation content of a Bambusa edulis xylem mutant, vse, derived from somaclonal variation
作者: Lin, C.S.
Lin, H.L.
Jane, W.N.
Hsiao, H.W.
Lin, C.C.
Jheng, F.Y.
Chang, W.C.
關鍵字: long-term subculture
iron
potassium
tissue culture
growth
plants
phosphorus
roots
期刊/報告no:: Journal of the American Society for Horticultural Science, Volume 131, Issue 4, Page(s) 445-451.
摘要: A xylem mutant (vse) was isolated from a Bambusa edulis (Odashima) Keng plantlet following vegetative micropropagation and subculture for 7 consecutive years and induced to proliferate in medium supplemented with 0.1 mg(.)L(-1) (0.5 mu m) thidiazuron (TDZ) and to develop roots in medium supplemented with 5 mg(.)L(-1) (26.9 mu m) alpha-naphthaleneacetic acid (NAA). Subsequent investigations comparing the growth habits of mutant plantlets with those of the wild type indicated that the growth of the former was retarded in a greenhouse. Several morphological abnormalities were observed in the vse mutant: it had thinner stems with fewer trichromes on the surface; the xylem vessels were smaller in diameter and contained crystal-like structures in the pith; the leaves were shorter and narrower with a sharp leaf blade angle; the roots were thinner and contained fewer xylem cells. The cation concentrations of both the mutant and wild type were similar in the in vitro analysis, except for those of iron and potassium, which were lower in mutant leaves in vivo. In 2-month-old mutant plants, iron chlorosis was observed on young leaves and a potassium deficiency was observed on older leaves. After 1 year of growth in the greenhouse, all of the wild-type plants had survived, but only 27% (16/60) of the mutant vse plants were alive.
URI: http://hdl.handle.net/11455/70110
ISSN: 0003-1062
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