Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/70186
標題: The central cavity from the (alpha/alpha)(6) barrel structure of Anabaena sp CH1N-acetyl-D-glucosamine 2-epimerase contains two key histidine residues for reversible conversion
作者: Lee, Y.C.
Wu, H.M.
Chang, Y.N.
Wang, W.C.
Hsu, W.H.
關鍵字: N-acetyl-D-glucosamine 2-epimerase
UDP-N-acetyl-D-glucosamine
2-epimerase
sialic acid
sugar-metabolizing enzyme
reversible
epimerization
d-glucosamine 2-epimerase
n-acetylneuraminic acid
acetyl-d-glucosamine
renin-binding protein
sialic-acid
angstrom
resolution
acetylglucosamine 2-epimerase
galactose mutarotase
glcnac
2-epimerase
crystal-structure
期刊/報告no:: Journal of Molecular Biology, Volume 367, Issue 3, Page(s) 895-908.
摘要: N-Acetyl-D-glcuosamine 2-epimerase (GlcNAc 2-epimerase) catalyzes the reversible epimerization between N-acetyl-D-glucosamine (GIcNAc) and Nacetyl-D-mannosamine (ManNAc). We report here the 2.0 angstrom resolution crystal structure of the GlcNAc 2-epimerase from Anabaena sp. CHI. The structure demonstrates an (alpha/alpha)(6) barrel fold, which shows structural homology with porcine GlcNAc 2-epimerase as well as a number of glycoside hydrolase enzymes and other sugar-metabolizing enzymes. One side of the barrel structure consists of short loops involved in dimer interactions. The other side of the barrel structure is comprised of long loops containing six short beta-sheets, which enclose a putative central active-site pocket. Site-directed mutagenesis of conserved residues near the N-terminal region of the inner alpha helices shows that R57, H239, E308, and H372 are strictly required for activity. E242 and R375 are also essential in catalysis. Based on the structure and kinetic analysis, H239 and H372 may serve as the key active site acid/base catalysts. These results suggest that the (alpha/ alpha)6 barrel represents a steady fold for presenting active-site residues in a cleft at the N-terminal ends of the inner alpha helices, thus forming a fine-tuned catalytic site in GlcNAc 2-epimerase. (c) 2006 Elsevier Ltd. All rights reserved.
URI: http://hdl.handle.net/11455/70186
ISSN: 0022-2836
文章連結: http://dx.doi.org/10.1016/j.jmb.2006.11.001
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