Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/70247
標題: The inhibitory effect of phenylpropanoid glycosides and iridoid glucosides on free radical production and beta 2 integrin expression in human leucocytes
作者: Lin, L.C.
Wang, Y.H.
Hou, Y.C.
Chang, S.
Liou, K.T.
Chou, Y.C.
Wang, W.Y.
Shen, Y.C.
關鍵字: promising antinephritic agent
endothelial-cells
human neutrophils
cistanche deserticola
adhesion molecule-1
calcium influx
sieboldii
miq
inflammation
acteoside
phenylethanoids
期刊/報告no:: Journal of Pharmacy and Pharmacology, Volume 58, Issue 1, Page(s) 129-135.
摘要: Rapid production of reactive oxygen species (ROS) and upregulation of beta 2 integrin by leucocytes are two important inflammatory responses in human leucocytes. To evaluate whether three phenylpropanoid glycosides (acteoside, crenatoside, and rossicaside B) and two iridoid glucosides (boschnaloside and 8-epideoxyloganic acid) identified from two medicinal plants with similar indications (Orobanche caerulescens and Boschniakia rossica) exhibited anti-inflammatory activity, their effects on N-formyl-methionyl-leucyl-phenylalanine (fMLP) and phorbol-12-myristate-13-acetate (PMA)-activated peripheral human neutrophils (PMNs) and mononuclear cells were examined. Pretreatment with 1-50 mu M phenylpropanoid glycoside concentration-dependently diminished PMA- and fMLP-induced ROS production with IC50 values of approximately 6.8-23.9 and 3.0-8.8 mu M, respectively. Iridoid glucoside was less effective than phenylpropanoid glycoside with an IC50 value of approximately 8.9-28.4 mu M in PMA-activated PMNs and 19.1-21.1 mu M in fMLP-activated mononuclear cells. Phenylpropanoid glycosides also effectively inhibited NADPH oxidase (NOX) and displayed potent free radical-scavenging activity, but did not interfere with pan-protein kinase C (PKC) activity. Furthermore, all compounds, except rossicaside B, significantly inhibited PMA- and fMLP-induced Mac-1 (a beta 2 integrin) upregulation at 50 mu M but not that of fMLP-induced intracellular calcium mobilization. These drugs had no significant cytotoxicity as compared with the vehicle control. Our data suggested that inhibition of ROS production, possibly through modulation of NOX activity and/or the radical scavenging effect, and beta 2 integrin expression in leucocytes indicated that these compounds had the potential to serve as anti-inflammatory agents during oxidative stress.
URI: http://hdl.handle.net/11455/70247
ISSN: 0022-3573
文章連結: http://dx.doi.org/10.1211/jpp.58.1.0016
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