請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/70394
標題: The formation of triple stranded helix of oligodeoxyribonucleotides containing 8-oxo-2 '-deoxyadanosine
作者: Lin, S.B.
Wu, C.M.
Lin, W.C.
Kan, L.S.
關鍵字: triple stranded helix (triplex)
oligodeoxyribonucleotide
8-oxo-2
'-deoxyadenosine
UV
fluorescence
protein-kinase-c
ft-ir spectroscopy
neutral ph
antisense
oligonucleotides
cytosine methylation
alpha
expression
2'-o-methylpseudoisocytidine
tumorigenicity
conformations
期刊/報告no:: Journal of the Chinese Chemical Society, Volume 46, Issue 5, Page(s) 693-697.
摘要: Triple stranded DNA helix (triplex) formation is limited by pH and the sequence of oligodeoxyribonucleotide. In this study, the 8-oxo-2'-deoxyadenosine (8-oxoA) can replace 2'-deoxycytidine for forming a base triad with a base pair guanine and cytosine. The target for the tripler formation is a motif of a promotor (5'-d-TTGGGGGGGAA, -3 - -12 of PKCalpha m-RNA) of mouse protein kinase C-alpha. The probes contain 20 bases with a common sequence 5'-d-XXXXXXTTCCCCCCAA. The XXXXXX portion may contain either 5-methyl-2'-deoxycytidines, six 8-oxoA's or both. Only the probe with a motif of six 8-oxoA's can for triplex with the target in the presence of magnesium ion and spermine at neutral pH. Therefore, 8-oxoA is useful for tripler formation under physiological conditions.
URI: http://hdl.handle.net/11455/70394
ISSN: 0009-4536
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