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標題: Liquid chromatography incorporating ultraviolet and electrochemical analyses for dual detection of zeranol and zearalenone metabolites in mouldy grains
作者: Hsieh, H.Y.
Shyu, C.L.
Liao, C.W.
Lee, R.J.
Lee, M.R.
Vickroy, T.W.
Chou, C.C.
關鍵字: zeranol
tandem mass-spectrometry
resorcylic acid lactones
bovine urine
期刊/報告no:: Journal of the Science of Food and Agriculture, Volume 92, Issue 6, Page(s) 1230-1237.
摘要: BACKGROUND: Zeranol (Z) is a semi-synthetic mycotoxin that is used in some countries as a growth-promoting agent in livestock. In view of the known oestrogenic actions by Z and certain Z analogues, significant concerns exist with regard to the presence of Z residues in human foods and the potential for untoward effects, including carcinogenicity within the reproductive system. In order to confirm that foods are free from harmful Z residues, regulators need a quick and reliable analytical method that can be used for routine confirmation of Z-positive samples identified by enzyme-linked immunosorbent assay (ELISA) screening. In this study the authors have developed and validated a simple and rapid high-performance liquid chromatography method incorporating ultraviolet (UV) absorbance (wavelength 274 nm) and electrochemical (EC) dual-mode detection for simultaneous determination of Z-related mycotoxins produced from mouldy grain matrices, including rice, soybean and corn flakes. RESULTS: Recoveries for all analytes were around 80% and the limits of detection ranged from 10 to 25 ngmL-1 for UV and from 50 to 90 ng mL(-1) for EC detection with good accuracy and reproducibility. Differential profiles and occurrence rates of Z, beta-zearalenol, beta-zearalanol and a-zearalenol in naturally moulded grain matriceswere observed, indicating differentmetabolite patterns and possibly grain-specific effects ofmycotoxin exposure for humans and animals. The strength of this dual detection method lies in its selectivity characterised by a carbon screen-printed electrode such that aflatoxin interference is precluded. CONCLUSION: The combined dual detection technique affords quick and reliable semiconfirmative and quantitative information on multiple types of Z analogues in mouldy grains without the necessity of using expensive mass spectrometry. The method is considered a superior supplement to ELISA, which only screens total Z immunoreactivity. (C) 2011 Society of Chemical Industry
ISSN: 0022-5142
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