請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/70755
標題: Baculovirus Transduction of Mesenchymal Stem Cells: In Vitro Responses and In Vivo Immune Responses After Cell Transplantation
作者: Chuang, C.K.
Wong, T.H.
Hwang, S.M.
Chang, Y.H.
Chen, G.Y.
Chiu, Y.C.
Huang, S.F.
Hu, Y.C.
關鍵字: mediated gene-transfer
toll-like receptors
transgene expression
bone-formation
progenitor cells
mammalian-cells
delivery vector
dendritic cells
stromal cells
cord blood
期刊/報告no:: Molecular Therapy, Volume 17, Issue 5, Page(s) 889-896.
摘要: Baculovirus holds great promise for the genetic modification of mesenchymal stem cells (MSCs). However, whether baculovirus transduction provokes undesired MSCs responses that might compromise their in vivo applications has yet to be examined. Hereby, we unraveled that baculovirus transduction of human MSCs upregulated the transcription of interleukin (IL)-1 beta, interferon (IFN)-alpha and IL-6, but not tumor necrosis factor (TNF)-alpha and IFN-gamma. However, only IL-6 secretion was detectable by enzyme-linked immunosorbent assay (ELISA). Baculovirus transduction also stimulated transient, low level upregulation of human leukocyte antigen I (HLA-I) on the human MSCs surface, yet it did not either altered the HLA-II expression or impaired the MSCs ability to inhibit lymphocyte proliferation. After transplantation into allogeneic rats, the transduced rat MSCs elicited transient, mild macrophage responses, but the cells remained tolerant as judged by the persistence of transplanted cells and absence of CD8(+) T cells infiltration. Besides, transplantation of the transduced MSCs did not provoke systemic induction of monocytes and CD8(+) T cells. This study, for the first time, explores the responses of MSCs to virus transduction and confirms the safety of transplanting baculovirus-engineered MSCs into immunocompetent animals for cell-based gene therapy.
URI: http://hdl.handle.net/11455/70755
ISSN: 1525-0016
文章連結: http://dx.doi.org/10.1038/mt.2009.30
顯示於類別:期刊論文

文件中的檔案:
沒有與此文件相關的檔案。


在 DSpace 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。