請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/70802
標題: Curcumin-Induced DNA Damage and Inhibited DNA Repair Genes Expressions in Mouse-Rat Hybrid Retina Ganglion Cells (N18)
作者: Lu, H.F.
Yang, J.S.
Lai, K.C.
Hsu, S.C.
Hsueh, S.C.
Chen, Y.L.
Chiang, J.H.
Lu, C.C.
Lo, C.
Yang, M.D.
Chung, J.G.
關鍵字: Curcumin
DNA damage
DNA repair
N18 mouse-rat hybrid retina ganglion
cells
human breast-cancer
induced apoptosis
gel-electrophoresis
lipid-peroxidation
alzheimers-disease
crude extracts
pathway
nuclear
mitochondrial
cytotoxicity
期刊/報告no:: Neurochemical Research, Volume 34, Issue 8, Page(s) 1491-1497.
摘要: Curcumin is reported to be a potent inhibitor of the initiation and promotion of many cancer cells. We investigated to examine whether or not curcumin induce DNA damage in mouse-rat hybrid retina ganglion cell line N18 cells. The Comet assay showed that incubation of N18 cells with 10, 25 and 30 mu M of curcumin led to a longer DNA migration smear (Comet tail). The DNA gel electrophoresis showed that 20 mu M of curcumin for 24 and 48 h treatment induced DNA damage and fragments in N18 cells. The real time PCR analysis showed that 20 mu M of curcumin for 48 h treatment decreased ATM, ATR, BRCA1, 14-3-3 sigma, DNA-PK and MGMT mRNA, and ATM and MGMT mRNA expression were inhibited in a time-dependent manner. Our results indicate that curcumin caused DNA damage and inhibited DNA repair genes which may be the factors for curcumin-inhibited cell growth.
URI: http://hdl.handle.net/11455/70802
ISSN: 0364-3190
文章連結: http://dx.doi.org/10.1007/s11064-009-9936-5
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