請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/70804
標題: Danthron Induces DNA Damage and Inhibits DNA Repair Gene Expressions in GBM 8401 Human Brain Glioblastoma Multiforms Cells
作者: Lu, H.F.
Lai, T.Y.
Hsia, T.C.
Tang, Y.J.
Yang, J.S.
Chiang, J.H.
Lu, C.C.
Liu, C.M.
Wang, H.L.
Chung, J.G.
關鍵字: Danthron
DNA damage
Comet assay
DNA repair
Human brain glioblastoma
multiforms GBM 8401 cells
mitochondria-dependent pathways
cancer scc-4 cells
induced apoptosis
alzheimers-disease
breaks
curcumin
n18
期刊/報告no:: Neurochemical Research, Volume 35, Issue 7, Page(s) 1105-1110.
摘要: Our primary studies had shown that danthron induced cytotoxic effects, including apoptosis and inhibition of migration and invasion. However, danthron-affected DNA damage and repair gene expressions are not clear. In this study, we investigated to examine whether or not danthron induced DNA damage and inhibited DNA repair gene expression in human brain glioblastoma multiforms (GBM 8401) cells. The results from Comet assay indicated that incubation of GBM 8401 cells with 0, 50, 100 and 150 mu M of danthron led to a longer DNA migration smear based on the single cell electrophoresis (Comet tail). The results from real-time PCR assay demonstrated that 100 mu M of danthron for 24 h treatment in GBM 8401 cells led to decrease all examined ataxia telangiectasia mutated (ATM), ataxia-telangiectasia and Rad3-related (ATR), breast cancer 1, early onset (BRCA-1), 14-3-3 proteins sigma (14-3-3 sigma), DNA-dependent serine/threonine protein kinase (DNA-PK) and O (6) -methylguanine-DNA methyltransferase (MGMT) mRNA expressions. Taken together, the present study showed that danthron caused DNA damage and inhibited DNA repair genes, which may be the factors for danthron-inhibited cell growth in vitro.
URI: http://hdl.handle.net/11455/70804
ISSN: 0364-3190
文章連結: http://dx.doi.org/10.1007/s11064-010-0161-z
顯示於類別:期刊論文

文件中的檔案:
沒有與此文件相關的檔案。


在 DSpace 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。