Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/70811
標題: Effect of naloxone on the induction of immediately early genes following oxygen- and glucose-deprivation in PC12 cells
作者: Huang, H.M.
Yu, J.Y.
Ou, H.C.
Jeng, K.C.
關鍵字: oxygen- and glucose-deprivation
immediate early genes
naloxone
ERK
c-fos
transcription factors
nitric-oxide
rat-brain
in-vitro
hypoxia
expression
jun
neurons
activation
期刊/報告no:: Neuroscience Letters, Volume 438, Issue 2, Page(s) 252-256.
摘要: Cerebral ischemia/reperfusion involves inflammatory process and naloxone is able to reduce infarct volume and has been used as a therapeutic agent for brain injury. Hypoxia induces the immediate early genes (IEGs) rapidly and transiently that may initiate a cascade of cellular responses that are necessary for survival and normal function. However, the protective effect of naloxone on ischemic/hypoxic neuronal cells was only partly studied. Thus, the effects of naloxone on oxygen- and glucose-deprivation (OGD) and OGD followed by reoxygenation. (OGD/R) on the expression of IEGs were examined in PC12 cells. The result showed that lactate dehydrogenase (LDH) released in the media was reduced by naloxone. The temporal response of IEG mRNA encoding c-fos, c-jun, nur77, and zif268 was induced with different degree of intensity following hypoxia, whereas the level of GAPDH mRNA was relatively constant. However, these signals of c-fos, c-jun, and nur77 by hypoxia were reduced significantly by naloxone. Treatment with OGD also activated mitogen-activated protein kinase (MAPK) pathway. The induction of c-fos, c-jun, nur77, and zif268 by hypoxia was inhibited by naloxone (0.1 mu M) and MAPK inhibitors (10 mu M of U0126, D98059, SB203580). However, naloxone increased the expression of ERK1/2 by OGD concomitantly diminished the LDH release. Thus, the present studies demonstrated that OGD induced IEGs including c-fos, c-jun, nur77, and zif268 and MAPK signaling pathways were regulated differently by naloxone. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
URI: http://hdl.handle.net/11455/70811
ISSN: 0304-3940
文章連結: http://dx.doi.org/10.1016/j.neulet.2008.04.036
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