Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/70981
標題: Direct Observation of Multiple Pathways of Single-Stranded DNA Stretching
作者: Chen, W.S.
Chen, W.H.
Chen, Z.P.
Gooding, A.A.
Lin, K.J.
Kiang, C.H.
關鍵字: atomic-force microscope
fluorescence spectroscopy
secondary structure
b-dna
sequence
molecules
proteins
rna
recognition
polymerase
期刊/報告no:: Physical Review Letters, Volume 105, Issue 21.
摘要: We observed multiple pathways of stretching single-stranded polydeoxynucleotides, poly(dA). Poly(dA) has been shown to undergo unique transitions under mechanical force, and such transitions were attributed to the stacking characteristics of poly(dA). Using single-molecule manipulation studies, we found that poly(dA) has two stretching pathways at high forces. The previously observed pathway has a free energy that is less than what is expected of single-stranded DNA with a random sequence, indicating the existence of a novel conformation of poly(dA) at large extensions. We also observed stepwise transitions between the two pathways by pulling the molecule with constant force, and found that the transitions are cooperative. These results suggest that the unique mechanical property of poly(dA) may play an important role in biological processes such as gene expression.
URI: http://hdl.handle.net/11455/70981
ISSN: 0031-9007
文章連結: http://dx.doi.org/10.1103/PhysRevLett.105.218104
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