Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/71032
標題: The promoter for the maize C-4 pyruvate,orthophosphate dikinase gene directs cell- and tissue-specific transcription in transgenic maize plants
作者: Taniguchi, M.
Izawa, K.
Ku, M.S.B.
Lin, J.H.
Saito, H.
Ishida, Y.
Ohta, S.
Komari, T.
Matsuoka, M.
Sugiyama, T.
關鍵字: C-4 photosynthesis
maize (Zea mays)
promoter
pyruvate,orthophosphate
dikinase
transgenic plant
bundle-sheath-cells
ortho-phosphate dikinase
nadp-malic enzyme
orthophosphate dikinase
phosphoenolpyruvate carboxylase
agrobacterium-tumefaciens
differential expression
mesophyll-cells
zea-mays
pyruvate
期刊/報告no:: Plant and Cell Physiology, Volume 41, Issue 1, Page(s) 42-48.
摘要: The pyruvate, orthophosphate dikinase (PPDK) gene coding the chloroplast enzyme involved in C-4 photosynthesis has a dual promoter system, The first promoter is responsible for the transcription of a larger transcript and its product is targeted to the chloroplast (hence, it is designated as C4Pdk promoter) while the second promoter is responsible for the transcription of a smaller transcript and its product remains in the cytosol. In this study, chimeric maize C4Pdk promoter (0.9 or 1.5 kb)-beta-glucuronidase or luciferase fusion genes were introduced into maize plants by Agrobacterium-mediated transformation. The cell- and tissue-specificities of the maize C4Pdk promoter in the transgenic maize plants were examined by histochemical and enzymic activity analyses of the reporters in different photosynthetic cells and tissues. The results showed that the reporter proteins are almost exclusively localized in leaf mesophyll cells, Among the tissues tested, leaf blade had the highest reporter activities with sheath exhibiting about 10% of the activities in blade. Husk, stem, tassel and root had no or very little reporter activities. Taken together, these results suggest that the maize C4Pdk promoter is specifically transcribed in the mesophyll cells of leaf blade and to a much less extent in the mesophyll cells of sheath, but not in leaf bundle sheath cells or other tissues. Furthermore, the 0.9 kb maize C4Pdk promoter sequences appear to contain the necessary cis-acting elements for its cell- and organ-specific expression.
URI: http://hdl.handle.net/11455/71032
ISSN: 0032-0781
Appears in Collections:期刊論文

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