Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/71037
標題: Development of insect-resistant transgenic cauliflower plants expressing the trypsin inhibitor gene isolated from local sweet potato
作者: Ding, L.C.
Hu, C.Y.
Yeh, K.W.
Wang, P.J.
關鍵字: cauliflower
Brassica oleracea var. botrytis L.
trypsin inhibitor
insect resistance
Agrobacterium mediated transformation
l var botrytis
agrobacterium-tumefaciens
shoot regeneration
transformation
explants
期刊/報告no:: Plant Cell Reports, Volume 17, Issue 11, Page(s) 854-860.
摘要: Agrobacterium-mediated transformation was used to introduce a trypsin inhibitor gene into Taiwan cauliflower (Brassica oleracea var, botrytis L.) cultivars. The TI gene was isolated from a well-adapted Taiwan sweet potato cultivar and was expected to be especially effective in combating local pests. In vitro regeneration studies indicated that 4-day-old cauliflower seedling hypocotyl segments, pretreated with 2,4-dichlorophenoxyacetic acid for 3 days and incubated on a silver-ion-containing shoot induction medium, gave regeneration rates greater than 95%. Optimum transformation conditions were determined. G418 selection at 15 mg/l was initiated 1 week after cocultivation, and the dose was doubled 1 week later. Over 100 putative transgenic plants were produced. Transgenic status was confirmed by in vitro TI activity, and Southern and Western hybridization assays. The transgenic plants demonstrated in planta resistance to local insects to which the control plants were vulnerable.
URI: http://hdl.handle.net/11455/71037
ISSN: 0721-7714
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